Protease S. aureus V8 (endoproteinase-Glu-C)Protease S. aureus V8 (also known as endoproteinase Glu C) is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping. It specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The